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KMID : 0613820030130010059
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Journal of Life Science
2003 Volume.13 No. 1 p.59 ~ p.66
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Effect of Lactic Acid Bacterium on Antioxidative and ACE inhibitory activity in Dolsan Leaf Mustard Kimchi
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Choi Myeong-Rak
Yoo Eun-Jeong
Lim Hyun-Soo
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Abstract
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The bacterial strain was isolated from the 4th day’s fermented Dolsan Leaf Mustard Kimchi(DLMK) at 20℃. It was used as Kimchi starter, and then its physiological activity was investigated for 50 days at 4℃ and 10℃. The physiological activity of DLMK was examined for both antioxidative and Angiotensine Converting Enzyme(ACE) inhibitory activity. In the starter-inoculated DLMK(1×1010 CFU/mL) at 4 and 10℃, the optimal ripening period was more shortend than that of control(without starter) up to about 5.6 and 5 times, respectively. The maximal antioxidative activity in the starter-inoculated DLMK(1×1010 CFU/mL) at 4 and 10℃ were 67% and 75%, respectively. The yield of cell concentration per day(lnXmax/tmax) and the yield of antioxidative activity per day(Pmax/tmax) had a linear relationship. Also, the yield of antioxidative activity per day was increased with increasing the concentration of
inoculated bacterium. By adding 1×1010 CFU/mL at 4 and 10℃, the ACE inhibitory activity of DLMK was maximal. The rates of inhibiting activities were 52% and 76%, respectively. Consequently, physiological activities were significantly affected by the inoculation concentrations of starter, but bacterium itself was not appeared the physiological activity. We assume that the bacterium metabolizes certain materials in DLMK and released compounds such as glucosinolates or its metabolized forms from DLMK show the antioxidative and ACE inhibitory activity.
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KEYWORD
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DLMK, starter, ACE, physiological activity
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